![]() No sequences are imported (or only some, but not all sequences are imported).Sequencher appears to have problems when trying to import sequences and trace files which have long file names.This is a confusing problem, because Sequencher often does not give any error messages - just some things are wrong after importing. ![]() FASTA files (and also not in the chromat_dir or the same directory as the Phred-generated SCf files). ![]() Make sure that the input files for Phred are NOT in the directory above the.QUAL files in a directory called edit_dir, and SCF files in a sister directory called chromat_dir). Make sure that all files created by Phred are either all in the same directory, or in directories that follow the structure required by Consed (FASTA and.When running Phred, make sure to create (a) a single FASTA file, (b) a single FASTA.QUAL file, and (c) SCF files. ![]() Ideally, use only letters, numbers, and underscores.
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